Record Display for the EPA National Library Catalog


Main Title Natural Transformation of a Marine 'Vibrio' Species by Plasmid DNA.
Author Jeffrey, W. H. ; Paul, J. H. ; Stewart, G. J. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;University of South Florida, St. Petersburg.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/377;
Stock Number PB91-163907
Additional Subjects Vibrio ; Water microbiology ; Marine biology ; Deoxyribonucleic acids ; Plasmids ; Chromosomes ; Southern blotting ; Autoradiography ; Reprints ; Genetic transformation
Library Call Number Additional Info Location Last
NTIS  PB91-163907 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
A series of thirty marine and estuarine bacterial isolates was examined for the ability to naturally transform with plasmid DNA. One isolate from Tampa Bay, Florida, identified as Vibrio parahaemolyticus, successfully incorporated and maintained the broad host range plasmid pKT230 in both filter transformation assays and sterile sediment microcosms, at frequencies ranging from 0.3 to 3.1 x 10 to the minus eighth power transformants per recipient. Transformation occurred without deletions and transformation of multimeric forms of the plasmid. Results suggest that natural transformation may be one mechanism by which estuarine bacteria acquire antibiotic resistance plasmids.