Main Title |
Field Calibration of Soil Core Microcosms for Evaluating Fate and Effects of Genetically Engineered Microorganisms in Terrestrial Ecosystems. |
Author |
Bolton, H. ;
Fredrickson, J. K. ;
Bentjen, S. A. ;
Workman, D. J. ;
Li, S. W. ;
|
CORP Author |
Battelle Pacific Northwest Labs., Richland, WA.;Corvallis Environmental Research Lab., OR.;Department of Energy, Washington, DC. |
Publisher |
Apr 91 |
Year Published |
1991 |
Report Number |
DE-AC06-76RLO-1830; EPA/600/3-91/041; |
Stock Number |
PB91-216366 |
Additional Subjects |
Terrestrial ecosystems ;
Soil microbiology ;
Genetic engineering ;
Pseudomonas ;
Seasonal variations ;
Field tests ;
Temperature ;
Soil water ;
Microbial colony count ;
Risk assessment ;
Soil-core microcosms ;
Streptomyces lividans
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-216366 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
88p |
Abstract |
Pacific Northwest Laboratory compared intact soil-core microcosms and the field for ecosystem structural and functional properties after the introduction of a model genetically engineered microorganism (GEM). The project used two distinct microbial types as model GEMs, Gram-negative Pseudomonas sp RC1, which was an aggressive root colonizer, and Gram-positive Streptomyces lividans TK24. The model GEMs were added to surface soil in separate studies throughout a ten month period. Also, RC1 was used in studies conducted during two consecutive field seasons to determine how year-to-year field variability influenced the calibration of microcosms with the field. The main conclusions of the research were that intact soil-core microcosms can be useful to simulate the field for studies of microbial fate and effects on ecosystem structural and functional properties. |