| Main Title |
Seed germination and root elongation toxicity tests in hazardous waste site evaluation : methods development and applications / |
| Author |
Linder, Greg. ;
Greene, J. C. ;
Ratsch, H. ;
Nwosu, J. ;
Smith, S.
|
| Other Authors |
|
| CORP Author |
Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. |
| Publisher |
U.S. Environmental Protection Agency, Environmental Research Laboratory, |
| Year Published |
1989 |
| Report Number |
EPA/600/D-89/109; ERL-COR-1038D |
| Stock Number |
PB90-113184 |
| Additional Subjects |
Toxicity ;
Germination ;
Plants(Botany) ;
Hazardous materials ;
Waste disposal ;
Mutagenicity tests ;
Soil pollutants ;
Superfund program ;
Environmental monitoring
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-113184 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
34 pages ; 28 cm |
| Abstract |
Seed germination tests measure soil toxicity directly, while root elongation tests consider the indirect effects of water-soluble constituents which may be present in site-samples. In the seed germination toxicity test, site-soil is mixed with a reference soil to yield exposure concentrations into which test seeds are planted. Germination is evaluated after a five-day exposure, and effective concentrations associated with a 50% reduction in seed germination are calculated. Root elongation tests evaluate site-soil eluates. Here seeds are placed onto moistened filter paper which lines Petri dish exposure chambers; exposure chambers are then covered and incubated in complete darkness for five days. Inhibition of root elongation is calculated as an EC50 (exposure concentration which yields a 50% reduction in root length relative to controls) upon termination of the test. |
| Notes |
"Paper presented at the First Symposium on Use of Plants in Toxicity Assessment, April 16-21, Atlanta, GA, 1989." Includes bibliographical references. "EPA/600/D-89/109"--Cover. Microfiche. |
