Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Evaluation of urban runoff and combined sewer overflow mutagenicity /
Other Authors
Author Title of a Work
Spiegel, Stuart J.
Publisher U.S. Environmental Protection Agency, Municipal Environmental Research Laboratory,
Year Published 1984
Report Number EPA/600-S2-84-116
OCLC Number 11614543
Subjects Sewage--Environmental aspects--United States.
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 600-S2-84-116 In Binder Headquarters Library/Washington,DC 11/28/2017
EJBD  EPA 600-S2-84-116 In Binder Headquarters Library/Washington,DC 08/13/2018
Collation 5 pages ; 28 cm
Caption title. At head of title: Project summary. "Sept. 1984." Distributed to depository libraries in microfiche. "EPA/600-S2-84-116."
Contents Notes
"A study was conducted to evaluate combined sewer overflows and urban runoff for the presence of chemical mutagens. The Ames Salmonella/microsome mutagenicity test was used as a general biological effects test for the qualitative detection of mutagens in the sanitary environment, including rain, urban runoff, sanitary wastewater, combined sewer overflows, sewage treatment plant effluent, and receiving waters. The Ames test is a relatively sensitive and simple bacterial test for detecting chemical mutagens. Its advantages over long-term animal tests are speed, ease, and relatively low cost. The test employs previously mutated Salmonella typhimurium LT2 bacterial strains that tend to mutate back to their natural state when exposed to mutagenic compounds. Nineteen samples produced a detectable response to one or more of the five S. typhimurium test strains, with or without metabolic activation. Nine of these samples (47%) were of urban runoff in the project area of metropolitan Syracuse (Onondaga County), New York, and they produced 17 of 30 detectable responses (57%). Five of the samples (26%) were from combined sewer overflows, and they produced 7 of 30 detectable responses (23%). The results indicated that urban runoff components that produce a detectable response in the Ames test may be diluted or inactivated in combination with sanitary sewage to form combined sewage, since fewer responses were detected in the latter than in urban runoff."