||Preparation of Research Reagents for 2 Dengue Strains.
Shope., Robert E. ;
||Yale Univ., New Haven, Conn. Yale Arbovirus Research Unit.;National Institutes of Health, Bethesda, Md.;National Inst. of Allergy and Infectious Diseases, Bethesda, Md. Research Resources Branch.
Dengue virus ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Four lots totalling 3,050 ml of dengue 2 mouse immune ascitic fluid; 5 lots totalling 3,365 ml of dengue 3 immune ascitic fluid; and two lots of 200 ml each of control ascitic fluids were prepared. Plaque reduction neutralization tests indicated that most lots of dengue 2 fluids had homologous neutralization about 32-fold greater than heterologous. Dengue 3 fluids were less specific, with homologous titers 4-8 fold greater than heterologous. Seed virus reagents for dengue 2 and dengue 3 viruses had titers of 9.0 log LD50/ml and 7.0 log LD50/ml respectively. Control ascitic fluids did not neutralize dengue viruses.