Record Display for the EPA National Library Catalog


Main Title Transcription factor protocols /
Other Authors
Author Title of a Work
Tymms, Martin J.
Publisher Humana Press,
Year Published 2000
OCLC Number 40489061
ISBN 0896035735; 9780896035737
Subjects Transcription factors--Laboratory manuals ; Transfection--méthodes ; Analyse de séquence d'ADN--méthodes ; Facteurs de transcription--analyse ; Wetenschappelijke technieken ; Séquenðcage des acides nucléiques ; Transcription Factors--analysis--https://idnlmnihgov/mesh/D014157Q000032 ; Sequence Analysis, DNA--methods--https://idnlmnihgov/mesh/D017422Q000379 ; Transfection--methods--https://idnlmnihgov/mesh/D014162Q000379
Internet Access
Description Access URL
Publisher description
Library Call Number Additional Info Location Last
ELDM  QP552.T68T727 2000 CCTE/GLTED Library/Duluth,MN 08/11/2000
Collation x, 306 pages : illustrations ; 23 cm.
Includes bibliographical references and index.
Contents Notes
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.