Record Display for the EPA National Library Catalog


Main Title Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher U.S. Environmental Protection Agency, Office of Water,
Year Published 2002
Report Number PB2002-108489; EPA-821-R-02-013
Stock Number PB2002-108489
OCLC Number 52856537
Subjects Water quality bioassay--United States ; Toxicity testing--United States ; Effluent quality--United States
Additional Subjects Test methods ; Toxicity testing ; Effluents ; Water pollution ; Concentration(Composition) ; Water quality ; Standards ; Algae ; Aquatic animals ; Freshwater biology ; Daphnia ; Fathead minnow ; NPDES(National Pollutant Discharge Elimination System) ; Ceriodaphnia dubia ; Selenastrum capricornutum ; Pimephales promelas
Internet Access
Description Access URL
Local Library Info
Library Local Subject Local Note
EJD Printed from the Selected Office of Water methods and guidance version 4 CD-ROM.
Library Call Number Additional Info Location Last
EJDD  EPA-821-R-02-013 Env Science Center Library/Ft Meade,MD 08/15/2003
ELBD ARCHIVE EPA 821-R-02-013 AWBERC Library/Cincinnati,OH 03/06/2023
ESAD  EPA 821-R-02-013 Region 10 Library/Seattle,WA 09/20/2010
NTIS  PB2002-108489 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Edition 4th ed.
Collation xiv, 335 pages : illustrations ; 28 cm
The objective of aquatic toxicity tests with effluents or pure compounds is to estimate the 'safe' or 'no effect' concentration of these substances, which is defined as the concentration which will permit normal propagation of fish and other aquatic life in the receiving waters. The endpoints that have been considered in tests to determine the adverse effects of toxicants include death and survival, decreased reproduction and growth, locomotor activity, gill ventilation rate, heart rate, blood chemistry, histopathology, enzyme activity, olfactory function, and terata. Since it is not feasible to detect and/or measure all of these (and other possible) effects of toxic substances on a routine basis, observations in toxicity tests generally have been limited to only a few effects, such as mortality, growth, and reproduction. This manual describes chronic toxicity tests for use in the National Pollutant Discharge Elimination System (NPDES) Permits Program to identify effluents and receiving waters containing toxic materials in chronically toxic concentrations. The methods included in this manual are referenced in Table IA, 40 CFR Part 136 regulations and, therefore, constitute approved methods for chronic toxicity tests. They are also suitable for determining the toxicity of specific compounds contained in discharges. The tests may be conducted in a central laboratory or on-site, by the regulatory agency or the permittee.
"October 2002." Includes bibliographical references. "EPA-821-R-02-013."