Record Display for the EPA National Library Catalog


Main Title Recovery and Diversity of Heterotrophic Bacteria from Chlorinated Drinking Waters.
Author Maki, J. S. ; LaCroix, S. J. ; Hopkins, B. S. ; Staley, J. T. ;
CORP Author Washington Univ., Seattle. Dept. of Microbiology and Immunology.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab.
Year Published 1986
Report Number EPA/600/J-86/165;
Stock Number PB87-114625
Additional Subjects Bacteria ; Potable water ; Reprints ; Water pollution detection ; Drinking water ; Seattle(Washington)
Library Call Number Additional Info Location Last
NTIS  PB87-114625 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Heterotrophic bacteria were enumerated from the Seattle drinking water catchment basins and distribution system. The highest bacterial recoveries were obtained by using a very dilute medium containing 0.01% peptone as the primary carbon source. Other factors favoring high recovery were the use of incubation temperatures close to that of the habitat and an extended incubation (28 days or longer provided the highest counts). Total bacterial counts were determined by using acridine orange staining. With one exception, all acridine orange counts in chlorinated samples were lower than those in prechlorinated reservoir water, indicating that chlorination often reduces the number of acridine orange-detectable bacteria. Source waters had higher diversity index values than did samples examined following chlorination and storage in reservoirs. Shannon index values based upon colony morphology were in excess of 4.0 for prechlorinated source waters, whereas the values for final chlorinated tap waters were lower than 2.9.