||Rotavirus Fecal IgA Antibody Response in Adults Challenged with Human Rotavirus.
Bernstein, D. I. ;
Ziegler, J. M. ;
Ward., R. L. ;
||James N. Gamble Inst. of Medical Research, Cincinnati, OH.;Health Effects Research Lab., Research Triangle Park, NC.
Viral antibodies ;
Rotavirus infections ;
Enzyme-linked immunosorbent assay
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Studies of rotavirus challenge in adult volunteers made it possible to evaluate the relationship of preexisting anti-rotavirus fecal IgA antibody to infection and illness and to investigate the local response to this infection. No relationship could be found between the preexisting levels of fecal anti-rotavirus IgA antibody and protection from infection or illness. A > 6-fold increase in the level of antibody was seen in 16/19 infected volunteers with determinable increases but not in 0/15 controls who received less than the minimal infectious dose of rotavirus. Antibody levels increased rapidly in infected volunteers and were consistent with an anamnestic response. Two of seven volunteers who received an infectious dose of rotavirus but were considered uninfected on the basis of other laboratory methods had greater than or equal to 6-fold rises of fecal antibody and one of these experienced symptoms compatible with a rotavirus infection. This finding indicates that an increase in fecal antibody may be a reliable indicator of rotavirus infection even in the absence of detectable shedding or seroconversion. (Copyright (c) 1986 Alan R. Liss, Inc.)