Record Display for the EPA National Library Catalog


Main Title Discussion Summary: Application of Biotechnology to the Systematics and Monitoring of Biocontrol Agents.
Author Kawanishi, C. Y. ; Hemming, B. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Monsanto Agricultural Co., St. Louis, MO. Technology Div.
Publisher 1989
Year Published 1989
Report Number EPA/600/D-89/149;
Stock Number PB90-112764
Additional Subjects Deoxyribonucleic acids ; Bacteria ; Enterobacteriaceae ; Biotechnology ; Gene amplification ; Ribosomal RNA
Library Call Number Additional Info Location Last
NTIS  PB90-112764 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The report is a general discussion of the application of biotechnology to the systematics and monitoring of microbial agents used in biotechnology. Present conventional methods such as microscopy, selective media, marker utilization, etc., were described. Two newer methods, rRNA sequencing and DNA amplification techniques were described and their usefulness in identifying and monitoring microbes used in biotechnology discussed. These were concluded to be the most useful new techniques. Automated methods for bacteria identification and quantification were discussed. These methods were shown to be in need of adaptation to agricultural agents. The relevance of these methodologies is to the Agency's regulation of biotechnology products.