Record Display for the EPA National Library Catalog


Main Title Phylogenetic Tree of 16S rRNA Sequences from Sulfate-Reducing Bacteria in a Sandy Marine Sediment.
Author Devereux, R. ; Mundfrom, G. W. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. Microbial Ecology and Biotechnology. ;Technical Resources, Inc., Gulf Breeze, FL.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/436;
Stock Number PB95-111829
Additional Subjects Phylogeny ; Sulfate-reducing bacteria ; Marine biology ; 16S ribosomal RNA ; Sediments ; Aquatic microbiology ; Sequence alignment ; Ribosomal DNA ; Oligonucleotide probes ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB95-111829 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Phylogenetic divergence among sulfate-reducing bateria in an estuarine sediment sample was investigated by PCR amplification and comparison of partial 16S rDNA sequences. Twenty unique 16S rDNA sequences were found, 12 from delta subclass bacteria based on overall sequence similarity (82-91%). Two successive PCR amplifications were used to obtain and clone the 16S rDNA. The first reaction used templates derived from phosphate-buffered saline washed sediment with primers designed to amplify nearly full-length bacterial domain 16S rDNA. A produce from a first reaction was used as template in a second reaction with primers designed to selectivity amplify a region of 16S rDNA genes of sulfate-reducing bacteria. A phylogenetic tree incorporating the cloned sequences suggests the presence of yet to be cultivated lines of sulfate-reducing bacteria within the sediment sample.