||Simplified Radioimmunoassay for Detection of Human Rotavirus in Stools.
Cukor, George ;
Berry, Mary Kay ;
Blacklow, Neil R. ;
||Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Cincinnati, OH.
||EPA-R-805169 ;EPA-R-806591-01-0; EPA/600/J-78/184;
Electron microscopy ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
A simplified radioimmunoassay (RIA) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. This microtiter solid-phase RIA utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. One hundred thirteen pediatric stool samples were tested. Thirty-five specimens known to be positive for rotavirus by electron microscopy were also positive by RIA. All RIA-positive reactions (36) were shown to be specific for rotavirus by means of an antibody-blocking test. Nonspecific RIA-positive reactions were not encountered. This accurate, sensitive RIA technique is practical for use in diagnostic laboratories because critical serum reagents are prepared against culture-grown virus and not against rotavirus derived from carefully selected stools of humans or experimentally infected gnotobiotic animals.