Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Immune Response and Prevalence of Antibody to Norwalk Enteritis Virus as Determined by Radioimmunoassay.
Author Blacklow, Neil R. ; Cukor, George ; Bedigian, Mary Kay ; Echeverria, Peter ; Greenberg, Harry B. ;
CORP Author Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1981
Report Number EPA-R-805169 ;EPA-R-806591; EPA/600/J-79-128;
Stock Number PB81-232209
Additional Subjects Enteroviruses ; Viruses ; Antibodies ; Reprints ; Immune responses ; Radioimmunoassay
Library Call Number Additional Info Location Last
NTIS  PB81-232209 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 9p
A solid-phase microtiter radioimmunoassay was established for the detection of Norwalk virus and its antibody, with clinical materials from human volunteers previously studied in Massachusetts as reagents. A study of 308 Massachusetts residents showed that serum antibody to Norwalk agent was rarely present during childhood but was detectable in approximately 50% of adults. All volunteers inoculated with Norwalk virus who developed illness seroconverted (10/10), whereas only one-third (5/15) of nonill volunteers seroconverted (P=0.0009). The 10 nonill, nonseroconverting subjects had undetectable to low preexisting antibody levels. Paradoxically, 10/13 subjects with preexisting antibody became ill, whereas 17/25 lacking antibody did not (P=0.009). All 3 subjects with preexisting anti-Norwalk radioimmunoassay blocking activity in duodenal intraluminal fluids became ill, whereas only 5/11 lacking such activity developed illness (P=0.15). These data further support the unique concept that some individuals are susceptible to repeated infections with this agent, whereas others are incapable of developing infection.