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Main Title Time-Dependent Effects of Acute Chlorpyrifos Administration on Spatial Delayed Alternation and Cholinergic Neurochemistry in Weanling Rats.
Author Stanton, M. E. ; Mundy, W. R. ; Ward, T. ; Dulchinos, V. ; Barry, C. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
Publisher Apr 94
Year Published 1994
Report Number EPA/600/J-94/529;
Stock Number PB95-148912
Additional Subjects Neurochemistry ; Dursban ; Pesticides ; Toxicology ; Animal behavior ; Weaning ; Rats ; Acetylcholinesterase ; Quinuclidinyl benzilate ; Tritium ; Radioligand assay ; Hippocampus ; Cerebral cortex ; Dose-response relationships ; Memory ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB95-148912 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
On postnatal day 21 (PND21), Long-Evans rat pups received a single subcutaneous injection of either O (corn oil vehicle), 90, 120, or 240 mg/kg chlorpyriphos and were then tested for T-maze delayed alternation on PND23 or 26. Acetylcholinesterase (AChE) activity and muscaranic receptor density (i.e., quinuclidinyl benzilate (QNB) binding) were determined in hippocampus and cortex of brains taken from pups 15 hours after the end of behavioral testing (i.e., the morning of PND24, and 27). Pups exposed to the 240 mg/kg dose of chlorpyrifos showed signs of overt toxicity that precluded behavioral testing. Exposure to the 120 mg/kg dose produced a selective memory impairment (i.e., a deficit in delayed alternation but not position discrimination) relative to the 90 mg/kg and vehicle groups. This impairment was transient, however, as it appeared on PND23 and was absent by PND26. PND21 exposure to chlorpyrifos produced dose-related inhibition and recovery of brain AChE over the PND24-27 age range. A similar pattern was observed in hippocampus. Binding of ((3)H)QNB was reduced in frontal cortex on PND27 only at the 240 mg/kg dose. No significant effects were observed in the hippocampus. These results suggest that the neurochemical effects of acute chlorpyrifos administration are more transient, and the behavioral effects are smaller and shorter-lived than what has been reported in adult rats. (Copyright (c) 1993 Intox Press, Inc.)