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Main Title Age-Related Changes in Receptor-Mediated Phosphoinositide Hydrolysis in Various Regions of Rat Brain.
Author Mundy, W. ; Tandon, P. ; Ali, S. ; Tilson, H. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;National Center for Toxicological Research, Jefferson, AR.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/210;
Stock Number PB91-243014
Additional Subjects Aging(Biology) ; Phosphoinositides ; Brain ; Rats ; Hydrolysis ; Cerebral cortex ; Corpus striatum ; Quinuclidinyl benzilate ; Muscarinic receptors ; Inositol phosphates ; Myoinositol ; Carbachol ; Norepinephrine ; Quisqualic acid ; Choline acetyltransferase ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-243014 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
The effects of age on cholinergic markers and receptor-stimulated phosphoinositide hydrolysis was examined in the frontal cortex and striatum of male Fischer-344 rats. Choline acetyltransferase activity was decreased 27% in the striatum of aged (24 month) rats compared to young (3 month) controls. Muscarinic receptor density as measured by (3)H-quinuclidinyl benzilate binding showed a similar 26% decrease in the striatum of aged rats. Phosphoinositide hydrolysis was measured by the release of inositol phosphate (IP) from tissue slices prelabeled with (3)H myoinositol in response to carbachol, norepinephrine, and quisqualate. In the cortex, stimulated IP release was significantly greater in slices from aged rats compared to young rats for all three agonists. In contrast, stimulated IP release was significantly decreased in striatal slices from aged rats compared to young for all three agonists. These data indicate a differential effect of age on agonist-stimulated phosphoinositide hydrolysis in the cortex and striatum. The decreased responsiveness in the latter area may result from the age-related loss of postsynaptic receptors.