Record Display for the EPA National Library Catalog
RECORD NUMBER: 27 OF 120| Main Title | Differences in Detection of DNA Adducts in the 32P-Postlabelling Assay After Either 1-Butanol Extraction or Nuclease Pl Treatment. | |||||||||||
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| Author | Gallagher, J. E. ; Jackson, M. A. ; George, M. H. ; Lewtas, J. L. ; Robertson., I. G. ; | |||||||||||
| CORP Author | Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. | |||||||||||
| Publisher | c1989 | |||||||||||
| Year Published | 1989 | |||||||||||
| Report Number | EPA/600/J-89/471; | |||||||||||
| Stock Number | PB91-115972 | |||||||||||
| Additional Subjects | Butanols ; Mutagens ; Chemical analysis ; Nitrogen organic compounds ; Aromatic hydrocarbons ; Solvent extraction ; Sensitivity ; Reprints ; Mutagenicity tests ; DNA damage ; Deoxyribonucleases ; Phosphorus 32 ; Benzo(a)pyrene ; Autoradiography | |||||||||||
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| Collation | 8p | |||||||||||
| Abstract | The use of nuclease P1 treatment and 1-butanol extraction to increase the sensitivity of the 32P-postlabelling assay for DNA adducts have been compared. Although similar results were obtained with the two methods for standard adducts formed with benzo(a)pyrene diol epoxide I, nuclease P1 treatment resulted in a significant reduction in detection of major adducts 1-amino-6-nitropyrene, 1-amino-8-nitropyrene, 2-aminofluorene, 2-naphthylamine and 4-aminobiphenyl modified DNAs, but not following the 32P-postlabelling analysis of 2-acetylaminofluorene modified DNA. These results suggest that at least initially, both modications of the 32P-postlabelling assay should be used for the detection of unknown adducts or for adducts derived from nitro-aromatics and aromatic amines. (Copyright (c) 1989 Elsevier Science Publishers Ireland Ltd.) |