Record Display for the EPA National Library Catalog


Main Title Increase in Angiotensin-Converting Enzyme in Rat Lungs Following Inhalation of Phosgene.
Author Jaskot, R. H. ; Grose, E. C. ; Stead, A. G. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA-68-02-4450; EPA/600/J-89/463;
Stock Number PB91-109546
Additional Subjects Lung ; Toxicology ; Phosgene ; Ozone ; Rats ; Epithelium ; Reprints ; Air pollution effects(Animals) ; Kininase II ; Organ weight ; Bronchoalveolar lavage fluid ; Pulmonary alveoli ; Macrophages ; Enzyme activation ; Endothelium
Library Call Number Additional Info Location Last
NTIS  PB91-109546 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Angiotensin converting enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II (a potent vasopressor) and plays an important role in homeostasis of blood pressure. ACE has been shown to be associated primarily with the luminal surfaces of lung vascular endothelial cells making it a possible target for phosgene and ozone. Sixty-day-old male Sprague Dawley rats were exposed to 0.5 ppm phosgene, 1.0 ppm ozone, or air for 4 hr. Rats were sacrificed immediately and 24 hr. postexposure. ACE activity was measured in lung, lavage fluid, lavage fluid cells, and serum. Both phosgene and ozone exposed rats showed no changes in ACE activity in either lavage fluid or serum compared to air controls. Whole lung ACE activity was significantly higher immediately and marginally higher 24 hours postexposure to phosgene with increases of 34% and 16%, respectively. Phosgene exposed rats also showed a significant increase in ACE activity in the lavage fluid cells. These increases were 50% and 54% above controls at 0 and 24 hr postexposure, respectively. The cell type most likely affected was the alveolar macrophage. No significant effects were seen following ozone exposure.