Record Display for the EPA National Library Catalog


Main Title Growth Inhibition of 'Cryptococcus neoformans' by Human Alveolar Macrophages (Journal Version).
Author Weinberg, P. B. ; Becker, S. ; Granger, D. L. ; Koren, H. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. ;Environmental Monitoring and Services, Inc., Chapel Hill, NC.
Publisher c1987
Year Published 1987
Report Number EPA/600/J-87/430;
Stock Number PB89-105886
Additional Subjects Crytacoccus neoformans ; Macrophages ; Phagocytosis ; Immunologic cytotoxicity ; Blood serum ; Pulmonary alveoli ; Humans ; Cultured cells ; Immune system ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB89-105886 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Macrophage cytotoxicity for Cryptococcus neoformans was investigated by culturing human alveolar macrophage (AM) with a thin-capsuled clone of C. neoformans. Under appropriate conditions, fungal replication was inhibited in the presence of human AM. The effect persisted over the 48-h time course that was evaluated. Human AM did not require endotoxin, fetal calf serum, or specific rabbit anticryptococcal antibody for fungistasis. Under these conditions, microscopic evaluation of a cytocentrifuge preparation of AM-yeast cocultures, stained by a modified Giemsa technique, revealed all the fungi to be extracellular. In the presence of 10 percent fresh human serum, AM phagocytized C. neoformans and exhibited fungicidal activity. Tumor necrosis factor did not affect the replication rate of the yeast. These findings suggest that there may be at least 2 mechanisms by which human AM protect against C. neoformans. One is serum-independent and extracellular and results in fungistasis, and the other is dependent on a serum factor and leads to intracellular inhibition of growth and possibly killing of the organism.