Record Display for the EPA National Library Catalog


Main Title Endogenously-Mediated, Pretranslational Suppression of Cytochrome P4501A in PCB-Contaminated Flounder.
Author Elskus, A. A. ; Pruell, R. ; Stegeman, J. J. ;
CORP Author Woods Hole Oceanographic Institution, MA. ;Marine Biological Lab., Woods Hole, MA.;Environmental Research Lab., Narragansett, RI.
Publisher c1992
Year Published 1992
Report Number EPA-R813155 ;PHS-ES04220; EPA/600/J-94/002 ; ERLN-1322
Stock Number PB94-140787
Additional Subjects Flat fishes ; Chlorinated organic compounds ; Pollution ; Sex ; Maturation ; Biphenyl ; Chlorine organic compounds ; Females ; Males ; Hormones ; Concentration(Composition) ; Steroids ; Reprints ; Cytochrome P4501A ; Flounder ; Polychlorinated biphenyl ; Pseudopleuronectes americanus ; Inducers
Library Call Number Additional Info Location Last
NTIS  PB94-140787 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Gonadally mature fish display strong sex-related differences in the content and activity of P4501A, the major polynuclear aromatic hydrocarbon-inducible P450 form in teleosts. Such differences appear related to plasma levels of the female sex steroid, estradiol (E2); however, neither the mechanism of estradiol suppression of P4501A nor the capacity for hormonal regulation to overcome P4501A induction by high concentrations of potent inducers are known. Gonadally mature flounder (Pseudopleuronectes americanus) were collected from Fox Island (FI), Rhode Island, a reference site, and New Bedford Harbor (NB), Massachusetts, a site highly contaminated with polychlorinated biphenyls. Differences in flounder P4501A expression were determined at the level of P4501A catalytic activity (measured as ethoxyresorufin-O-deethylase, EROD), P4501A protein content (immunoquantitated), and P4501A mRNA content (by Northern blot) as they relate to sex, reproductive status and hepatic PCB content. (Copyright (c) 1992 Elsevier Science Publishers Ltd, England.)