| Main Title |
Cloning, Expression, and Regulation of the 'Pseudomonas cepacia' Protocatechuate 3,4-Dioxygenase Genes. |
| Author |
Zylstra, G. J. ;
Olsen, R. H. ;
Ballou, D. P. ;
|
| CORP Author |
Michigan Univ., Ann Arbor. Medical School.;Environmental Research Lab., Gulf Breeze, FL. |
| Publisher |
cNov 89 |
| Year Published |
1989 |
| Report Number |
EPA/600/J-89/435; |
| Stock Number |
PB90-264771 |
| Additional Subjects |
Pseudomonas ;
Mutations ;
Reprints ;
Molecular cloning ;
Gene expression ;
Protocatechuate 3-4-dioxygenase ;
Bacterial genes ;
Plasmids ;
Restriction mapping
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-264771 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Genes for the alpha and beta subunits of the enzyme protocatechuate 3,4-dioxygenase were cloned from the Pseudomonas cepacia DB01 chromosome on a 9.5 kilobase pair PstI fragment into the broad-host-range cloning vector pR02317. The resultant clone was able to complement protocatechuate 3,4-dioxygenase mutations in P. cepacia, P. aeruginosa, and P. putida. Expression studies showed that the genes were constitutively expressed and subject to catabolite repression in the heterologous host. Since the cloned genes exhibited normal induction patterns when present in P. cepacia DB01 it was concluded that induction was subject to negative control. (Copyright (c) 1989 American Society for Microbiology). |
