Record Display for the EPA National Library Catalog


Main Title Characterization of the 'Pseudomonas aeruginosa recA' Analog and Its Protein Product: 'rec-102' is a Mutant Allele of the 'P. aeruginosa' PAO 'recA' Gene.
Author Kokjohn, T. A. ; Miller, R. V. ;
CORP Author Stritch School of Medicine, Maywood, IL. Dept. of Biochemistry and Biophysics.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA-R-812494; EPA/600/J-87/143;
Stock Number PB88-148994
Additional Subjects Pseudomonas aeruginosa ; Escherichia coli ; Deoxyribonucleic acids ; Mutagens ; Microbiology ; Tables(Data) ; Reprints ; Genetic intervention ; Recombinant proteins
Library Call Number Additional Info Location Last
NTIS  PB88-148994 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Cloned was a 2.3 kilobase pair fragment of the Pseudomonas aeruginosa PAO chromosome which is capable of complementing recA mutations of Escherichia coli. The recA-complementing activity was further localized to a 1.5 kilobase pair PvuII-HindIII fragment. The direction of transcription was determined. Southern analysis under conditions of high stringency indicated that DNA sequence homology is shared by the E. coli recA gene and the P. aeruginosa recA analogue. The cloned recA analogue was shown to restore resistance to methyl methane-sulfonate, nitrofurantoin and ultraviolet irradiation to E coli recA mutants. (Copyright (c) 1987, American Society for Microbiology.)