Record Display for the EPA National Library Catalog

RECORD NUMBER: 22 OF 339

Main Title Binding of Polychlorinated Biphenyls Classified as Either Phenobarbitone-, 3-Methylcholanthrene- or Mixed-Type Inducers to Cytosolic Ah Receptor.
Author Bandiera, A. ; Safe, S. ; Okey, A. B. ;
CORP Author Guelph Univ. (Ontario). Guelph-Waterloo Centre.;Environmental Research Lab.-Duluth, MN.
Year Published 1982
Report Number EPA-R-809764; EPA-600/J-82-369;
Stock Number PB83-240788
Additional Subjects Enzymes ; Proteins ; Chemical bonds ; Binding ; Laboratory animals ; Environmental surveys ; Toxicity ; In vitro analysis ; Chlorine organic compounds ; Liver ; Rats ; Public health ; Reprints ; Polychlorinated biphenyls ; Toxic substances ; Cholanthrene/methyl,Phenobarbitone
Holdings
Library Call Number Additional Info Location Last
Modified		 Checkout
Status
NTIS  PB83-240788 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 23p
Abstract
It has been postulated that reversible, high-affinity binding of 3-methyl-cholanthrene (MC)-type inducers to a receptor protein (the Ah receptor) in hepatic cytosol is essential for induction of aryl hydrocarbon hydroxylase (AHH) enzymic activity. To test this postulate, the binding affinities of 16 highly purified, synthetic chlorinated biphenyl (PCB) congeners, which have been categorized either as phenobarbitone (PB)-, MC- or mixed (PB + MC)-type inducers of cytochrome P-450-dependent monooxygenases have been examined. The affinity of individual biphenyl congeners for the receptor was determined by their competition with 2,3,7,8-(3 sup H) tetrachlorodibenzo-p-dioxin ((3 sup H)TCDD) for specific cytosolic binding sites as measured by sucrose density gradient analysis following dextran-charcoal treatment.