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Main Title Application of a Novel Radioimmunoassay to Identify Baculovirus Structural Proteins that Share Interspecies Antigenic Determinants.
Author Smith, Gale E. ; Summers, Max D. ;
CORP Author Texas A and M Univ., College Station. Dept. of Entomology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-R-805232; EPA-600/J-81-518;
Stock Number PB82-153776
Additional Subjects Viruses ; Proteins ; Identifying ; Antigens ; Labeled substances ; Immunology ; Virology ; Reprints ; Radioimmunoassay ; Staphylococcus aureus ; Baculoviruses
Library Call Number Additional Info Location Last
NTIS  PB82-153776 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Viral proteins were electrophoresed in polyacrylamide gels, transferred to nitrocellulose, and incubated with viral antisera, and the antibodies were detected with 125 I-labeled Staphylococcus aureus protein A. Antisera were prepared to purified and intact virions from five baculoviruses: Autographa californica, Porthetria dispar, Trichoplusia ni, and Heliothis zea nuclear polyhedrosis viruses (NPVs) and T. ni granulosis virus (GV). These antisera were tested against the virion structural polypeptides of 17 different spcies of baculoviruses. Specific multiple-nucleocapsid NPV (MNPV), single-nucleocapsid NPV (SNPV), and GV virion polypeptides were shown to have similar antigenic determinants and thus be immunologically related. The molecular weights of the virion polypeptides with cross-reacting antigenic determinants were identified. Antisera prepared to purified A. californica and H. zea MNPV polyhedrin (the occlusion body protein from NPVs) recognized antigenic determinants on all the polyhedrins and granulins (occlusion body protein from GVs) that were tested. No immunological relationship was detected between A. californica MNPV polyhedrin and any of the A. californica MNPV virion structural polypeptides present on either the virus isolated from occlusion bodies or A. californica MNPV extracellular virus from infected-cell cultures.