Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 4

Main Title

Reoviruses in water pollution testing : final report /

Author

Spendlove, Rex S. ; Barnett, B. B. ; George, D. B. ; Adams, D. J. ; Ridinger, D. N.

CORP Author

Utah State Univ., Logan.;Health Effects Research Lab., Research Triangle Park, NC.

Publisher

U.S. Environmental Protection Agency,

Year Published

1984

Report Number

EPA/600/1-84/022; EPA-R-806527

Stock Number

PB85-125847

Subjects

Reoviruses ; Sewage

Additional Subjects

Reoviruses ; Water pollution ; Tests ; Sewage ; Enzymes ; Recovery ; Bioassay ; Tolerances(Physiology) ; Protamines ; Precipitation(Chemistry) ; Evaluation ; Abundance ; Safety ; Rotoviruses

Holdings

Library	Call Number	Additional Info	Location	Last Modified	Checkout Status
NTIS	PB85-125847	Some EPA libraries have a fiche copy filed under the call number shown.		07/26/2022

Collation

59 pages ; 28 cm

Abstract

Twelve cell lines were tested to determine their sensitivity to reoviruses of three serotypes that had been isolated from sewage. Madin-Darby bovine kidney (MDBK) cells were the most susceptible. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count (ICC) and plaque assay procedures. Immunofluorescence assay is more sensitive and more rapid than plaque assay procedure for quantifying reoviruses recovered from sewage. A procedure for protamine sulfate precipitation of reoviruses and rotaviruses was developed. Some reoviruses recovered from sewage were inactivated by enzyme treatment. This report describes a study to evaluate reoviruses for their potential usefulness in water quality testing.

Notes

Caption title. "Final Report." "Nov. 1984." "EPA-600/1-84-022." Microfiche.