Record Display for the EPA National Library Catalog


Main Title Inhibin Increases and Progesterone Decreases Receptors for Gonadotropin-Releasing Hormone in Ovine Pituitary Culture.
Author Laws, S. C. ; Beggs, M. J. ; Webster, J. C. ; Miller., W. L. ;
CORP Author North Carolina State Univ. at Raleigh. Dept. of Biochemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/178;
Stock Number PB91-116889
Additional Subjects Progesterone ; Physiology ; Pituitary gland ; Sheep ; In vitro analysis ; Reprints ; Gonadotropin receptors ; Inhibin ; Cultured cells ; Competitive binding ; Dose-response relationships
Library Call Number Additional Info Location Last
NTIS  PB91-116889 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Treatments (48 h) with highly purified bovine or porcine inhibins (10 ng/ml) induced ovine pituitary cells to increase their binding for des-Gly(to the tenth power)-(D-Ala(to the sixth power))LHRH-ethylamide by 3.6- and 5-fold, respectively. Studies with less pure inhibin from porcine follicles showed that increased binding could reach 7-fold within 48 h and was due to higher numbers of receptors for GnRH. The 48-h increase in GnRH receptors was linear with time and was rapidly reversible, since removal of inhibin at 24 h decreased GnRH binding below control levels at 48 h. Inhibin (bovine or porcine) also increased GnRH-stimulated secretion of LH by 2-fold. The ED50 for both inhibin actions noted above was in the range of 0.5-2.0 ng/ml (in terms of highly purified bovine inhibin). Progesterone (P) totally counteracted inhibin induction of GnRH binding and GnRH-stimulated LH secretion at 48 h. (Copyright (c) 1990 by The Endocrine Society.)