Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Microcosm Method to Assess Survival of Recombinant Bacteria Associated with Plants and Herbivorous Insects (Journal Version).
Author Armstrong, J. L. ; Knudsen, G. R. ; Seidler, R. J. ;
CORP Author Corvallis Environmental Research Lab., OR. ;Oregon State Univ., Corvallis. Dept. of Microbiology.
Publisher c1987
Year Published 1987
Report Number EPA/600/J-87/455;
Stock Number PB89-134613
Additional Subjects Genetic intervention ; Recombinant DNA ; Plants(Botany) ; Ecosystems ; Population dynamics ; Survival ; Plasmids ; Reprints ; Pseudomonas cepacia ; Cutworms ; Peridroma saucia
Library Call Number Additional Info Location Last
NTIS  PB89-134613 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/08/1989
Collation 6p
A microcosm method was developed to investigate survival and fate of genetically engineered bacteria associated with plant surfaces and a plant-feeding insect, the variegated cutworm, Peridroma saucia. Larvae on radish plants in microcosms were sprayed with nonrecombinant Pseudomonas cepacia and a recombinant strain of P. cepacia carrying the transmissible plasmid R388::Tn1721. Immediately after spraying, P. cepacia comprised about 20%-30% of the total population on leaves. After 2 days, the total epiphytic population had increased approximately fourfold, while the P. cepacia strains had decreased to 2%-30% of their initial numbers. Plasmid transfer from P. cepacia R388::Tn 1721 to the nonrecombinant recipient P. cepacia strain was not observed. (Copyright (c) Springer-Verlag New York Inc. 1987.)