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RECORD NUMBER: 1 OF 52

OLS Field Name OLS Field Data

Main Title Absence of Mutations in Codon 61 of the HA-RAS Oncogene in Epithelia Cells Transformed In vitro by 7,12-Dimethylbenz(a)-Anthracene.

Author Mass, M. J. ; Austin, S. J. ;

CORP Author Environmental Protection Agency, Research Triangle Park, NC.

Publisher c1989

Year Published 1989

Report Number EPA/600/J-89/382;

Stock Number PB90-216177

Additional Subjects Mutations ; Epithelium ; Respiratory system ; In vivo analysis ; In vitro analysis ; Reprints ; Codons ; Oncogenes ; Dimethylbenzanthracene ; Transformed cell line ; Polymerase chain reaction ; Base sequence ; Ras genes

Holdings

Library	Call Number	Additional Info	Location	Last Modified	Checkout Status
NTIS	PB90-216177	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.		08/27/1990

Collation 7p

Abstract

Epithelial cells of the respiratory tract of rats were transformed in vitro by 7,12-dimethylbenz(a)anthracene (DMBA). Ha-ras exon 2 was amplified by the polymerase chain reaction and then sequenced directly. In 10 transformed cell lines, of which 5 are known to be tumorigenic, no mutations in the region of codon 59-61 were found. The results suggest that Ha-ras codon 61 mutations are not associated with cell transformation initiated with DMBA in this cell transformation system. These data imply other genes (oncogenes) are responsible for transformation of these cells. The results are discussed in relation to observations in various transformation systems in vivo and in vitro. (Copyright (c) 1989 Academic Press, Inc.)