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Main Title PCR cloning protocols /
Other Authors
Author Title of a Work
Chen, Bing-Yuan.
Janes, Harry W.
Publisher Humana Press,
Year Published 2002
OCLC Number 47756248
ISBN 0896039730; 9780896039735; 9781617372810; 1617372811; 9780896039698; 0896039692
Subjects Molecular cloning--Laboratory manuals ; Polymerase chain reaction--Laboratory manuals ; Polymerase kettingreactie ; Genetische manipulatie ; Wetenschappelijke technieken ; Kloneren ; Genklonierung--(DE-588)4123275-6 ; Methode--(DE-588)4038971-6 ; Polymerase-Kettenreaktion--(DE-588)4256726-9 ; Polymerase Chain Reaction--methods--https://idnlmnihgov/mesh/D016133Q000379 ; Cloning, Molecular--methods--https://idnlmnihgov/mesh/D003001Q000379 ; Polymerase Chain Reaction--methods--Laboratory Manuals ; Cloning, Molecular--methods--Laboratory Manuals ; Clonamiento molecular--Manual de laboratorio ; Clonage mol eculaire--Manuels de laboratoire ; R eaction en cha ine de la polym erase--Manuels de laboratoire
Internet Access
Description Access URL
Publisher description
Library Call Number Additional Info Location Last
ESBM  QH442.2.P37 2002 CPHEA/PESD Library/Corvallis,OR 12/12/2003
Edition 2nd ed.
Collation xiv, 439 pages : illustrations ; 23 cm.
Includes bibliographical references and index.
Contents Notes
Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch -- Computer programs for pcr primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen -- Single-step pcr optimization using touchdown and stepdown pcr programming / K.H. Roux -- Xl pcr amplification of long targets from genomic DNA / L.A. Kolmodin -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cdna fragments / J.T. Aatsinki -- Long distance reverse-transcription pcr / V. Thiel, J. Herold and S.G. Siddell -- Increasing pcr sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt -- Gc-rich template amplification by inverse pcr. DNA polymerase and solvent effects / A. Moreau [and others] -- Pcr procedure for the isolation of trinucleotide repeats / T. Tozaki -- Methylation-specific pcr / H. Ohashi -- Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance pcr and magnetic beads / X. Huang, Z. Yuan and X. Cao -- Cloning pcr products. An overview / B. Guo and Y. Bi -- Using t4 DNA polymerase to generate clonable pcr products / K. Wang -- Enzyme-free cloning of pcr products and fusion protein expression / B.A. Neilan and D. Tillett -- Directional restriction site-free insertion of pcr products into vectors / G.J. Chen -- Autosticky pcr. Directional cloning of pcr products with performed 5' overhangs / J. Gal and M. Kalman -- A rapid and simple procedure for direct cloning of pcr products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould -- Pcr approaches to DNA mutagenesis and recombination. An overview / B. Shen -- In-frame cloning of synthetic genes using pcr inserts / J.C. Pierce -- Megaprimer pcr / S. Barik -- Pcr method for generating multiple mutations at adjacent sites / J. Adamec -- A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone -- Pcr screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque -- Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold -- Random mutagenesis by whole-plasmid pcr amplification / D. Kim and F.P. Guengerich -- Pcr-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo -- Long distance vectorette pcr (ldv pcr) / J.A. Fenton, G. Pratt and G.J. Morgan -- Nonspecific, nested suppression pcr method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli -- Inverse pcr. Cdna cloning / S.H. Huang -- Inverse pcr. Genomic DNA cloning / A.Y. Jong [and others] -- Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong -- The isolation of DNA sequences flanking tn5 transposon insertions by inverse pcr / V.J. Martin and W.W. Mohn -- Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun -- Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored pcr / Z.N. Yang and T.E. Mirkov -- A "step down" pcr-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr -- Use of pcr in library screening. An overview / J. Zhu -- Cloning of homologous genes by gene-capture pcr / R. Mastrangeli and S. Donini -- Rapid and nonradioactive screening of recombinant libraries by pcr / M.W. King -- Rapid cdna cloning by pcr screening (rc-pcr) / T. Takumi -- Generation and pcr screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli -- Pcr-based screening for bacterial artificial chromosome libraries / Y. Yasukochi -- A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang -- A microtiter-plate-based high throughput pcr product purification method / R. Smith and K. Wang. Performing and optimizing PCR -- Mutagenesis and recombination -- Cloning unknown neighboring DNA -- Library construction and screening.