Record Display for the EPA National Library Catalog


Main Title Real-time PCR : current technology and applications /
Other Authors
Author Title of a Work
Logan, Julie
Edwards, Kirstin
Saunders, Nick
Publisher Caister Academic Press,
Year Published 2009
OCLC Number 227931709
ISBN 9781904455394; 1904455395
Subjects Polymerase chain reaction ; Real-Time Polymerase Chain Reaction--methods ; Biologie molâeculaire ; ADN = Acide dâesoxyribonuclâeique
Internet Access
Description Access URL
Table of contents
Library Call Number Additional Info Location Last
EJDM  QP606.D46R44 2009 Env Science Center Library/Ft Meade,MD 06/09/2014
Collation vii, 284 p., [4] p. of plates : ill. (some col.) ; 26 cm.
Includes bibliographical references and index.
Contents Notes
An overview of PCR platforms -- Homogeneous fluorescent chemistries for real-time PCR -- Reference gene validation software for improved normalization -- Data analysis software -- Performing real-time PCR -- Internal and external controls for reagent validation -- Introduction to the applications of real-time PCR -- Analysis of mRNA expression by real-time PCR -- Validation of array data -- Mutation detection by real-time PCR -- Real-time NASBA -- Application in clinical microbiology -- Diagnosis of invasive fungal infections -- Biodefence -- Real-time PCR: application to food authenticity and legislation -- Molecular haplotyping by real-time PCR. "This essential manual presents a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including: clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more. An essential book for all laboratories using PCR."--BOOK JACKET.