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Main Title PCR technology : current innovations /
Other Authors
Author Title of a Work
Nolan, Tania.
Bustin, Stephen A.,
Bustin, Stephen A..
Publisher CRC Press, Taylor & Francis,
Year Published 2013
OCLC Number 830683972
ISBN 9781439848050 (hardcover : alk. paper); 143984805X (hardcover : alk. paper)
Subjects Polymerase chain reaction--Methodology ; Polymerase Chain Reaction--methods ; Nucleic Acids--analysis ; Polymerase-Kettenreaktion--(DE-588)4256726-9
Library Call Number Additional Info Location Last
EJDM  QP606.D46P362 2013 Env Science Center Library/Ft Meade,MD 10/05/2015
Edition 3rd ed. /
Collation xxi, 435 p., [12] p. of plates : ill. (some col.) ; 27 cm.
Includes bibliographical references and index.
Contents Notes
"A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution"--Provided by publisher. Pt. 1. Template preparation -- pt. 2. Reaction components -- pt. 3. Instruments -- pt. 4. Design, optimization, QC, and standardization -- pt. 5. Data analysis -- pt. 6. Applications.