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Main Title Effect of a Base-Catalyzed Dechlorination Process on the Genotoxicity of PCB-Contaminated Soil.
Author DeMarini, D. M. ; Houk, V. S. ; Kornel, A. ; Rogers, C. J. ;
CORP Author Environmental Protection Agency, Research Triangle Park, NC. Office of Research and Development.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/433;
Stock Number PB93-141323
Additional Subjects Soil contamination ; Polychlorobiphenyl compounds ; Mutagens ; Catalysis ; Salmonella ; Escherichia coli ; Polyethylene glycol ; Sodium hydroxide ; Bacteriophages ; Reprints ; Dechlorination
Library Call Number Additional Info Location Last
NTIS  PB93-141323 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
We evaluated the genotoxicity of dichloromethane (DCM) extracts of PCB-contaminated soil before and after the soil had been treated by a base-catalyzed dechlorination process, which involved heating a mixture of the soil, polyethylene glycol, and sodium hydroxide to 250-350 C. This dechlorination process reduced by over 99% the PCB concentration in the soil, which was initially 2,200 ppm. The DCM extracts of both control and treated soils were not mutagenic in strain TA100 of Salmonella, but they were mutagenic in strain TA98. The base-catalyzed dechlorination process reduced the mutagenic potency of the soil by approximately one-half. The DCM extracts of the soils before and after treatment were equally genotoxic in a prophage-induction assay in E. coli, which detects some chlorinated organic carcinogens that were not detected by the Salmonella mutagenicity assay. These results show that treatment of PCB-contaminated soil by this base-catalyzed dechlorination process did not increase the genotoxicity of the soil.