Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Development of Immobilized Enzyme Systems for Enhancement of Biological Waste Treatment Processes.
Author Slot, Lawrence ;
CORP Author Grumman Aerospace Corp., Bethpage, N.Y.
Year Published 1970
Report Number DI-14-12-562; EPA-WQO-16050-DXN; 00002,; 16050-DXN-07/70
Stock Number PB-203 598
Additional Subjects ( Sewage treatment ; Activated sludge process) ; ( Enzymes ; Activated sludge process) ; Separation ; Lagoons(Ponds) ; Colloids ; Gels ; Bacteria ; Aeration ; Ribonucleic acids ; Amylase ; Lipase ; Polyacrylamides ;
Library Call Number Additional Info Location Last
NTIS  PB-203 598 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 67p
Degradation and utilization of waste water nutrients by microbial action is controlled by a complex enzyme system. In attempts to produce a faster-acting system, a method was developed to biochemically fractionate the microbial enzymes from activated sludge, to concentrate and characterize their activity, and to immobilize this activity by entrapment in a polyacrylamide gel. The enzyme gel preparation was tested for its effect on the biological degradation of a bench-scale activated sludge process. Soluble enzymatic components were readily separated from particulate cell components, and the remaining soluble system was fractioned such that catabolic activity in the enzyme systems of interest was maintained while non-essential components were removed. Immobilization within the gel allowed activity to be maintained during repeated washings, exposure to substrate, and storage. Limited bench-scale tests produced no meaningful results possibly on account of either incomplete polymerization of the gel, an improper activated sludge culture, or both.