Record Display for the EPA National Library Catalog

RECORD NUMBER: 4483 OF 4624

Main Title Use of the Spiral Salmonella Assay to Detect the Mutagenicity of Complex Environmental Mixtures.
Author Houk, V. S. ; Early, G. ; Claxton, L. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Saint Augustine's College, Raleigh, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/059;
Stock Number PB91-191668
Additional Subjects Mutagenicity tests ; Environmental pollutants ; Salmonella typhimurium ; Mixtures ; Exhaust emissions ; Solvents ; Metabolic activation ; Diesel fuels ; Reprints ; Wood smoke ; Coal combustion emissions
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB91-191668 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Abstract
Three sets of combustion emissions were selected for evaluation: automotive diesel exhaust, woodsmoke, and a coal combustion emission. Each sample was tested in the Salmonella mutagenicity assay according to standard protocol (plate incorporation) and spiral assay techniques. Two assays demonstrated the following: (1) Diesel exhaust was generally the most mutagenically potent sample in both assays. (2) Samples were more mutagenic on rev/microgram basis in the spiral assay, especially when metabolic activation was added. (3) The spiral assay required 1/20 the sample mass of the standard assay to test equivalent doses. (4) Dichloromethane extracts of the complex mixtures could be tested for mutagenicity in the spiral assay.