Record Display for the EPA National Library Catalog


Main Title Genetic Variation in Clonal Vertebrates Detected by Simple-Sequence DNA Fingerprinting.
Author Turner, B. J. ; Elder, J. F. ; Laughlin, T. F. ; Davis, W. P. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Virginia Polytechnic Inst. and State Univ., Blacksburg. Dept. of Biology.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/384;
Stock Number PB91-163972
Additional Subjects Vertebrates ; Evolution(Development) ; Genetics ; Fishes ; Measurement ; Deoxyribonucleic acids ; Variations ; Populations ; Divergence ; Histology ; Hybridization ; Comparison ; Breeding ; Reprints ; DNA fingerprinting ; Clonal diversity ; Poecilia formosa ; Rivulus marmoratus
Library Call Number Additional Info Location Last
NTIS  PB91-163972 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Measurement of clonal heterogeneity is central to understanding evolutionary and population genetics of roughly 50 species of vertebrates lack effective genetic recombination. A simple-sequence DNA fingerprinting with oligonucleotide probes (CAG)5 and (GACA)4 was used to detect heterogeneity in natural populations of two clonal fishes, Poecilia formosa, an apomictic unisexual, and Rivulus marmoratus, a selfing hermaphrodite. The technique clearly differentiates allozymically identical laboratory lines of R. marmoratus that were previously distinguishable only by histocompatibility analysis. The technique also revealed the first documented cases of apparent clonal turnover in a natural population of each species.