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RECORD NUMBER: 15 OF 24

Main Title Production of Monoclonal Antibodies to 'Legionella pneumophila' Serogroups 1 and 6.
Author Para, M. F. ; Plouffe, J. F. ;
CORP Author Ohio State Univ., Columbus. Div. of Infectious Diseases.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-R-812704; EPA/600/J-83/357;
Stock Number PB87-170130
Additional Subjects Antigens ; Lobar pneumonia ; Serology ; Immunology ; Epidemiology ; Isolation ; Diffusion ; Agglutination ; Reprints ; Legionella pneumophila ; Legionnaires disease ; Monoclonal antibodies
Holdings
Library Call Number Additional Info Location Last
Modified		 Checkout
Status
NTIS  PB87-170130 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract
To better define the surface antigens of Legionella pneumophila for clinical and experimental purposes, were produced monoclonal antibodies to L. pneumophila serogroups 1 and 6. Two hybridomas were produced in serogroup 1. One antibody, LP-I-17, recognized a serogroup-common antigen. The second antibody, LP-I-81, was specific for serogroup 1. Microagglutination assays of environmental and clinical isolates revealed a subgroup of serogroup 1 environmental isolates which were not agglutinated by LP-I-81. Immunodiffusion studies showed identity between the LP-I-81 antigen and the serogroup-specific antigen of serogroup 1 organisms. This antigen could be absorbed out of the serogroup 1 organism extract with LP-I-81-coated Staphylococcus aureus, leaving the serogroup-common antigens. Three hybridomas were produced to serogroup 6. All three produced antibodies which were serogroup 6 specific and agglutinated serogroup 6 bacteria. (Copyright (c) 1982, American Society for Microbiology.)