Record Display for the EPA National Library CatalogRECORD NUMBER: 7 OF 12
|Main Title||Letter from Gulf Oil Company to USEPA Regarding Information on the Mutation Assay and In Vitro Cell Transformation Testing Conducted at EG&G Mason Laboratories.|
|CORP Author||Gulf Refining and Marketing Co., Houston, TX.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.|
|Publisher||9 Feb 1981|
|Additional Subjects||Mutations ; Cells(Biology) ; Toxicology ; In vitro analysis ; Rats ; Lymphomas ; Metabolism ; Exposure(Physiology) ; Bioassay ; Cultures(Biology) ; Plates ; Laboratory tests ; US EPA ; Gulf Oil Company|
This test involves the growth of mice cells in culture dishes in the presence of the material being tested (the experimental protocol used is a modification of that described by Clive, D. and Spector, J.F.S., Laboratory Procedure for Assessing Specific Locus Mutations at the TK Locus in Cultured L5178Y Mouse Lymphoma Cells. Mutation Research 31:17-29, (1975)). After the 'exposure' period, the cells are washed and placed in a growth medium which restricts the growth of 'normal' cells. Only mutant cells should grow in this medium. Control groups are used to detect the growth of any non-exposed cells in the medium.