||Murine Cytomegalovirus Host Resistance Models.
Selgrade, M. J. K. ;
Daniels, M. J. ;
||Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Neutralization tests ;
Virus replication ;
Animal disease models ;
Natural killer cells ;
Plaque assay ;
Cytotoxic T-lymphocytes ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Mouse cytomegalovirus (MCMV) is a well developed and extremely useful and relevant host resistance model for immunotoxicity testing. Rat cytomegalovirus (RCMV) is currently under development and may have similar applications. Cytomegaloviruses are species specific; RCMV is a distinct virus from MCMV and both are distinct from human cytomegalovirus (HCMV). However, biologic and biophysical similarities between these viruses make the animal viruses good models for the human infection. The chapter discusses the rationale for using MCMV as a host resistance model, provides some representative data, and gives detailed information on methods needed to apply the model. Background on RCMV is also presented along with detailed methods (to the extent available) for applying the RCMV model are also provided.