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RECORD NUMBER: 12 OF 12

Main Title Use of Perifusion to Evaluate Hormonal Release In vitro from Rat Pituitary and Hypothalamic Tissue.
Author Goldman, J. M. ; Cooper., R. L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Publisher 1992
Year Published 1992
Report Number EPA/600/A-92/059;
Stock Number PB92-158534
Additional Subjects Pituitary hormones ; Hypothalamic hormones ; Physiology ; Toxicity ; Tissues(Biology) ; Drawings ; In vitro analysis ; Culture media ; Gonadorelin ; Laboratory animals ; Laboratory equipment ; Perifusion
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB92-158534 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 33p
Abstract
The use of in vitro procedures in reproductive toxicology has permitted a direct assessment of hormonal release from isolated tissue and a means by which to determine potential sites of toxicant insult. The present chapter describes a perifusion procedure that can be used to evaluate stimulated or baseline hormonal secretion from pituitary and brain hypothalamic tissue. The implementation and utility of various combinations of tissue stimulation are presented, along with a discussion of the relative advantages and limitations of perifusion as an experimental approach.