||Use of Perifusion to Evaluate Hormonal Release In vitro from Rat Pituitary and Hypothalamic Tissue.
Goldman, J. M. ;
Cooper., R. L. ;
||Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Pituitary hormones ;
Hypothalamic hormones ;
In vitro analysis ;
Culture media ;
Laboratory animals ;
Laboratory equipment ;
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The use of in vitro procedures in reproductive toxicology has permitted a direct assessment of hormonal release from isolated tissue and a means by which to determine potential sites of toxicant insult. The present chapter describes a perifusion procedure that can be used to evaluate stimulated or baseline hormonal secretion from pituitary and brain hypothalamic tissue. The implementation and utility of various combinations of tissue stimulation are presented, along with a discussion of the relative advantages and limitations of perifusion as an experimental approach.