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Main Title Development of an in vitro model for screening organophosphates for neurotoxicity (pilot study) /
Author Graham, Doyle G.
Other Authors
Author Title of a Work
Curley, August.
CORP Author Duke Univ. Medical Center, Durham, NC. Dept. of Pathology.;Health Effects Research Lab., Cincinnati, OH.
Publisher Health Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency,
Year Published 1980
Report Number EPA-600/1-80-016.; EPA-68-02-2953
Stock Number PB80-169428
OCLC Number 20699093
Subjects Organophosphorus compounds--Toxicology ; Organophosphorus Compounds--toxicity ; Nerve Degeneration
Additional Subjects Toxicology ; Phosphorus organic compounds ; In vitro analysis ; Models ; Development ; Toxicity ; Neuroblastoma ; Cell lines ; Tissue cultures ; Neurotoxins ; Norepinephrine ; Parathion ; Phosphorothioic acid/o-o-(dimethyl-ester)-o-(nitrophenyl-ester) ; Folex ; DEF-6 pesticide ; EPN insecticide ; Phasvel ; TOCOP pesticide
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 600-1-80-016 Headquarters Library/Washington,DC 04/24/2014
EKBD  EPA-600/1-80-016. Research Triangle Park Library/RTP, NC 10/05/2001
ELBD ARCHIVE EPA 600-1-80-016 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD  EPA 600-1-80-016 AWBERC Library/Cincinnati,OH 06/04/1999
NTIS  PB80-169428 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation iv, 36 p. : ill. ; 28 cm.
An in vitro model utilizing neuroblastoma cell lines was developed for the differentiation of organophosphorus compounds which have the potential for induction of delayed degeneration of peripheral nerves and the spinal cord. IMR-32 neuroblastoma cells, derived from a human tumor, showed specific (3H)-norepinephrine uptake. The greater effect of alkyl over aryl OP compounds suggests a relationship with water solubility, thus equilibrium between adsorption to agar and the surrounding medium or lysosomal contents. The effect of parathion was intermediate, less than that for merphos, DEF and EPN but greater than that for leptophos and TOCP. Electron microscopic observations suggest further studies to define a morphologic lesion peculiar to the clinically delayed neurotoxic OP compounds.
Includes bibliographical references (p. 33-35). "EPA-600/1-80-016". "February 1980". "Final Report". Interagency Contract No. 68-02-2953.