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Main Title Genetic Analysis of the aggA Locus Involved in Agglutination and Adherence of 'Pseudomonas putida', a Beneficial Fluorescent Pseudomonad.
Author Buell, C. R. ; Anderson, A. J. ;
CORP Author Utah Agricultural Experiment Station, Logan.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number PAPER-4152; EPA/600/J-93/219;
Stock Number PB93-205029
Additional Subjects Pseudomonas putida ; Bacterial genes ; Agglutination ; Open reading frames ; Bacterial adhesion ; Deoxyribonucleic acids ; Molecular sequence data ; Sequence deletion ; Genetic complementation test ; Glycoproteins ; Phenotype ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-205029 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
An isolate of Pseudomonas putida, which rapidly adheres to plant roots, is agglutinated by a glycoprotein from root surfaces. Agglutination is prevented and adherence to the root surface is diminished by Tn5 insertion in mutant 5123. Two cosmid clones from wild type P. putida and a 2.7-kbp EcoRI-HindIII subclone present in both cosmid clones restored agglutinability to wild type levels in transconjugants of the nonagglutinable (Agg-) Tn5 mutant 5123. These three clones increased agglutinability in transconjugants of the parental Agg+ isolate. The 2.7-kbp EcoRI-HindIII subclone restored adherence to bean root surfaces of 5123 to wild type levels in a short-term binding assay. Deletion analysis of the 2.7-kbp fragment indicated only 1.45 kbp was necessary for complementation of agglutinability in 5123. The sequence, termed the aggA locus, contains an open reading frame of 1,356 nucleotides encoding a predicted 50,509-Da protein. The distribution of the aggA locus in plant-associated bacteria, as detected through Southern hybridization, is limited to bacteria that express the agglutination phenotype.