Record Display for the EPA National Library Catalog


Main Title Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence.
Author Rice, E. W. ; Johnson, C. H. ; Clark, R. M. ; Fox, K. R. ; Reasoner, D. J. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Maryland Univ. at Baltimore. School of Medicine.;Department of Veterans Affairs Medical Center, Baltimore, MD.;Public Health Service, Rockville, MD.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/381; PHS-R01A128856;
Stock Number PB94-117504
Additional Subjects Vibrio cholerae ; Chlorine ; Virulence ; Cultured cells ; Phenotype ; Microbial colony count ; Microbial sensitivity tests ; Rabbits ; Bacterial adhesion ; pH ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-117504 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Vibrio cholerae 01 is able to shift between smooth and rugose colonial morphologies. Cultures of smooth V. cholerae strains were inactivated in less than 20 s at a concentration of 1.0 mg/l free chlorine. In contrast, cultures of rugose variants exposed to this concentration of chlorine showed an initial rapid drop in viable counts, followed by persistence of a protected subpopulation of cells. Viable V. cholerae could still be recovered from rugose cultures even after exposure to 2.0 mg/l free chlorine for 30 min. Preliminary studies suggest that resistance to killing by chlorine was due to formation of cell aggregates enclosed in a gelatinous mucoid material. Rugose strains appeared to be fully virulent, based on their ability to adhere to Caco-2 cells and elicit fluid accumulation in rabbit ileal loops. Our data suggest that the V. cholerae rugose phenotype represents a fully virulent survival form of the organism that can persist in the presence of free chlorine. (Copyright (c) 1993 Chapman & Hall.)