Record Display for the EPA National Library Catalog


Main Title Perinatal Immunotoxicity of Benzene Toward Mouse B Cell Development.
Author Wierda, D. ; King, A. ; Luebke, R. ; Reasor, M. ; Smialowicz, R. J. ;
CORP Author West Virginia Univ., Morgantown.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/331;
Stock Number PB90-199407
Additional Subjects Toxicology ; Embryos ; Benzene ; Bone marrow ; Mice ; Graphs(Charts) ; Liver ; Spleen ; Lipopolysaccharides ; Reprints ; B lymphocytes ; Phenotype ; Animal pregnancy ; Fluorescent antibody technic ; Cultured cells
Library Call Number Additional Info Location Last
NTIS  PB90-199407 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 18p
Benzene is widely used by chemical industries and exposure to benzene has been shown experimentally to be immunotoxic in adult animals. The present study addressed whether exposure of fetuses in utero to benzene compromises the development of fetal B lymphopoiesis and whether B-lymphocyte development recovers postnatally. Pregnant BALB/C dams were given intraperitoneal injections of benzene (100 mg/kg, twice daily) from day 12.5 of gestation through day 19.5 of gestation. Phenotypic analysis revealed that fetal liver cell suspensions from embryos exposed in utero contained fewer pre-B cells and B cells than corresponding controls. Fetal liver cell cultures established from these embryos also produced fewer B cells. In contrast, pre-B cells were elevated in the livers of 8-day-old neonates that had been exposed to benzene in utero. Moreover, responsiveness to the B-cell mitogen, LPS, was significantly decreased in spleen cell cultures derived from these neonates. The results indicate that in utero exposure to high concentrations of benzene alters fetal B lymphopoiesis and may compromise immune responsiveness postnatally.