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Main Title Biased DNA Integration in 'Colletotrichum gloeosporioides f. sp. aeschynomene' Transformants with Benomyl Resistance.
Author Armstrong, J. L. ; Harris, D. L. ;
CORP Author Corvallis Environmental Research Lab., OR.;ManTech Environmental Technology, Inc., Corvallis, OR.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/256;
Stock Number PB93-212629
Additional Subjects Microbial drug resistance ; Deoxyribonucleic acids ; Genetic transformation ; Benomyl ; Neurospora crassa ; Tubulin ; Nucleic acid hybridization ; Growth ; Phenotype ; Plasmids ; Virulence ; Reprints ; Colletotrichum gloeosporioides
Library Call Number Additional Info Location Last
NTIS  PB93-212629 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
A procedure is presented for transforming Colletotrichum gloeosporioides f. sp. aeschynomene to benomyl resistance by using a mutant beta-tubulin gene from Neurospora crassa. Hybridization between the N. crassa beta-tubulin gene and transformant DNAs digested with StyI indicated that the integration site in all transformants was in a specific region of the genome. Transformants tolerated up to 300 micrograms of benomyl per milliliter but differed in pigmentation, growth rate, and pathogenicity. All transformed strains remained benomyl resistant after repeated subculture on medium lacking benomyl. The authors speculate that the bias in the site of integration was due to selection against transformants with other configurations between the N. crassa beta-tubulin gene and C. g. aeschynomene genome, which were unstable, lethal, or unsuitable for expression of the benomyl phenotype. (Copyright (c) 1993 The American Phytopathological Society.)