Record Display for the EPA National Library Catalog


Main Title Biodegradation of Crystal Violet by the White Rot Fungus 'Phanerochaete chrysosporium'.
Author Bumpus, J. A. ; Brock, B. J. ;
CORP Author Utah State Univ., Logan.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-93/163;
Stock Number PB93-191609
Additional Subjects Gentian violet ; Biodeterioration ; Dyes ; Hydrogen peroxide ; Mass spectrometry ; Metabolism ; Lignin ; High pressure liquid chromatography ; Reprints ; Phanerochaete chrysosporium ; Lignin peroxidase
Library Call Number Additional Info Location Last
NTIS  PB93-191609 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Biodegradation of crystal violet (N,N,N',N',N',N''- hexamethylpararosaniline) in ligninolytic (nitrogen-limited) cultures of the white rot fungus Phanerochaete chrysosporium was demonstrated by the disappearance of crystal violet and by the identification of three metabolites (N,N,N',N',N'' -pentamethylpararosaniline, N,N,N',N'' -tetramethylpararosaniline, and N,N',N'' -trimethylpararosaniline) formed by sequential N-demethylation of the parent compound. Metabolite formation also occurred when crystal violet was incubated with the extracellular fluid obtained from ligninolytic cultures of this fungus, provided that an H2O2-generating system was supplied. This, as well as the fact that a purified ligninase catalyzed N-demethylation of crystal violet, demonstrated that biodegradation of crystal violet by this fungus is dependent, at least in part, upon its lignin-degrading system. In addition to crystal violet, six other triphenylmethane dyes (pararosaniline, cresol red, bromphenol blue, ethyl violet, malachite green, and brilliant green) were shown to be degraded by the lignin-degrading system of this fungus. (Copyright (c) 1988 American Society for Microbiology.)