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Main Title Effects of Selected Neuroactive Chemicals on Calcium Transporting Systems in Rat Cerebellum and on Survival of Cerebellar Granule Cells.
Author Kodavanti, P. R. S. ; Mundy, W. R. ; Tilson, H. A. ; Harry, G. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Div. of Intramural Research.
Publisher 18 Jun 93
Year Published 1993
Report Number EPA/600/J-94/071;
Stock Number PB94-141595
Additional Subjects Cerebellum ; Toxic substances ; Nervous system ; Calcium ; Rats ; Biological transport ; Ca(2+)-transporting ATPase ; Cell survival ; Lactate dehydrogenase ; Aluminum ; N-methyl aspartate ; Mitochondria ; Microsomes ; Chlorpromazine ; Reprints ; Permethrin ; Deltamethrin
Library Call Number Additional Info Location Last
NTIS  PB94-141595 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
This investigation examined the relationship between alteration of Ca(2+)-transport systems and cytotoxicity in vitro for a number of neuroactive chemicals including environmental pollutants. (45)Ca(2+) uptake as a measure of Ca(2+) sequestration was determined in mitochondria and microsomes isolated from cerebella of adult male Long-Evans hooded rats by differential centrifugation. Ca(2+) extrusion, measured as Ca(2+)-ATPase activity, was determined in synaptosomes prepared by sucrose density gradient. Cytotoxicity (lactate dehydrogenase leakage) was assessed in primary cultures of cerebellar granule cells from 6- to 8-day old Long-Evans rats. N-Methyl-D-aspartic acid (NMDA) did not alter synaptosomal Ca(2+)-ATPase activity or (45)Ca(2+) uptake in mitochondria and microsomes. However, chlorpromazine (CPZ), aluminum (Al), permethrin (PER), and deltamethrin (DEL) inhibited Ca(2+) sequestration by mitochondria and microsomes. Of all the chemicals tested, CPZ was the most potent in inhibiting Ca(2+)-transporting systems and was also cytotoxic.