Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Mutagenicity testing in mammalian cells : multiple drug-resistance markers /
Author Carver, June H. ; Adair, Gerald M. ; Wandres, Daniel L. ; Carver, J. H. ; Adair, G. M. ; Wandres, D. L.
Other Authors
Author Title of a Work
Rausa, G.
Adair, Gerald M.
Wandres, Daniel L.
CORP Author California Univ., Livermore. Lawrence Livermore Lab.;Environmental Protection Agency, Washington, DC.
Publisher Office of Energy, Minerals and Industry, U.S. Environmental Protection Agency,
Year Published 1979
Report Number EPA-600/7-79-173; UCID-18599; EPA-W-7405-eng-48
Stock Number PB80-183114
OCLC Number 53964424
Subjects Cell culture. ; Mammals. ; Mutagenicity testing.
Additional Subjects Mutations ; Cells(Biology) ; Mammals ; In vitro analysis ; Screening ; Tests ; Nitrogen organic compounds ; Sulfur organic compounds ; Mutagens ; Mutagenesis ; Cell lines ; Biological effects ; Imidazotriazine/amino ; Thioguanine ; Uridine/fluoro-deoxy ; Benzopyrenes ; Quabain ; Sulfonic acid/methyl ; Guanidine/N-methyl-N-nitro-nitroso ; Quinaline oxide/nitro ; Nitrosamine/dimethyl ; Mutagen screening ; Pollution ; Animal cells ; Cell cultures ; Evaluation ; Hamsters ; Performance testing ; ERDA/560301
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 600-7-79-173 Headquarters Library/Washington,DC 11/13/2013
EJED  EPA 600/7-79-173 OCSPP Chemical Library/Washington,DC 01/23/2004
EKBD  EPA-600/7-79-173 Research Triangle Park Library/RTP, NC 01/02/2004
ELDD  EPA-600/7-79-173 CCTE/GLTED Library/Duluth,MN 01/28/2005 DISPERSAL
ERAD  EPA 600/7-79-173 Region 9 Library/San Francisco,CA 02/11/2013
ESAD  EPA 600-7-79-173 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB80-183114 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
NTIS  UCID-18599 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation xiii, 61 pages : charts ; 28 cm.
Chinese hamster ovary (CHO) cell lines heterozygous at the adenine phosphoribosyltransferase (aprt) and thymidine kinase (tk) loci were isolated and used for single-step selection of spontaneous and induced mutants resistant to 8-azaadenine (AAr), 6-thioguanine (TGr), 5-fluorodeoxyuridine (FUdRr), or ouabain (OUAR). Mutation data are reported for direct mutagens ethyl methanesulfonate (EMS), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (NQO) and for promutagens dimethylnitrosamine (DMN) and benzo(a)pyrene (BaP) activated by rat liver homogenates. Critical plating densities were established for AAr, TGr, and FUdRr. Optimal expression of mutant phenotypes after mutation induction with EMS, DMN, or BaP were 2 to 4 d for AAr, 6 to 8 d for TGr, 3 d for OUAR, and 1 to 3 d for FUdRr. The induced mutant frequencies as a function of relative cell survival after treatment with EMS, DMN, or BaP showed locus-specific differences in sensitivity. Of 61 clonal isolates resistant to AA and assayed for APRT activity, 87% had < or = 5% wild type activity; of 30 TGr clones assayed, 83% had < or = 5% wild type HGPRT activity. Of 42 FUdRr clones assayed, 41 had <1% wild type TK activity. Fifty clones selected in medium containing FUdR displayed cross-resistance to 5-bromodeoxyuridine (BUdR) and trifluorothymidine (TFT) and all were sensitive to HAT (amethopterin-hypoxanthine-thymidine) medium. The tk locus showed the largest mutational response as a function of relative cell survival after mutagen treatment.
EPA Project Officer: G. Rausa. U.S.D.O.E. Project Director: G. Stapleton. Biomedical Sciences Division, Lawrence Livermore Laboratory, University of California. Contract Number: Dept. of Energy Contract Number W-7405-ENG-48 "August 1979." Includes bibliographical references (pages 52-60). "UCID-18599." "EPA-600/7-79-173."