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Main Title Up and down Procedure for Estimating Acute Oral Toxicity (ld50) in Rats with Cover Letter dated 08/21/1986.
CORP Author Hazleton Labs. America, Inc., Madison, WI.; Proctor and Gamble Co., Cincinnati, OH. Ivorydale Technical Center.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Year Published 1986
Report Number 86860000122
Stock Number OTS0510267
Additional Subjects Toxicology ; Health effects ; P-(tert-butyl)benzoic Acid (98-73-7) ; Acute Toxicity ; Mammals ; Rats ; Oral ; Gavage ; Toxic substances ; Laboratory animals ; CAS No 98-73-7
Library Call Number Additional Info Location Last
NTIS  OTS0510267 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 86p
Acute oral toxicity was evaluated in Sprague-Dawley rats administered p-tert-butyl benzoic acid using an up and down schedule. Mortality was observed in 1 out of 3 males receiving doses of 0.7 g/kg body weight, in 2 out of 2 males receiving doses of 0.9 g/kg, and in all 6 females receiving doses of 0.7 g/kg body weight. The LD50 value was determined to be 0.72 g/kg (95% confidence limits of 0.54 to 0.98 g/kg) in males; and was estimated to be less than 0.70 g/kg in females. Clinical observations included hypoactivity, ataxia, thin appearance, hunched posture, impaired use of front limbs, yellow-stained anal area, tremors, prostration, excess salivation, hypothermia, bradypnea, absence of pain reflex, spasticity, mydriasis, flaccidity, and respiratory congestion. One animal also exhibited cyanosis, absence of pain reflex, and a reddish material on its face and front paws. Gross necropsy of decedents and animals sacrificed at 7 days revealed: diffusely red glandular mucosa in stomachs; clear fluid in stomachs; enlarged stomach with clear red mucoid fluid; tan to dark tan mucoid material in intestines; red perinasal discharge; and enlarged right submandular lymph nodes.