Record Display for the EPA National Library Catalog


Main Title Catabolism of Aromatic Biogenic Amines by 'Pseudomonas aeruginosa' PA01 via meta Cleavage of Homoprotocatechuic Acid (Journal Version).
Author Cuskey, S. M. ; Olsen, R. H. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Michigan Univ., Ann Arbor. Medical School.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/109;
Stock Number PB89-120067
Additional Subjects Biogenic amines ; Pseudomonas aruginosa ; Metabolism ; Bacteria ; Tyramine ; Oxygenases ; NADH ; NADPH oxidoreductases ; Analklyamines ; Mutations ; Enzyme induction ; Molecular cloning ; Chromosome mapping ; Reprints ; Octopamine ; Benzene methanol/(aminomethyl)-hydroxy
Library Call Number Additional Info Location Last
NTIS  PB89-120067 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Pseudomonas aruginosa PA01 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). Meta ring cleavage was mediated by 3-4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-dependent dehydrogenase caused the disappearance of the yellow MSA product, probably forming 2-hydroxy-5-carboxymethylmuconic acid. Induction studies with extracts from mutant cells indicated that the inducer of HPADO was HPA and/or MSA. Strains PA01.221 (tynCl) and PA01.303 (tynDI) have chromosomal mutations causing a deficiency in the activity necessary for conversion of 4-hydroxyphenylacetic acid to HPA. (Copyright (c) 1988 American Society for Microbiology.)