Record Display for the EPA National Library Catalog


Main Title Role of mixed function oxidases in insecticide action /
Author Metcalf, Robert L. ; Metcalf, Robert Lee,
CORP Author Illinois Univ. at Urbana-Champaign. Dept. of Entomology.;Health Effects Research Lab., Research Triangle Park, N.C.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory ; Available through the National Technical Information Sercive,
Year Published 1976
Report Number EPA-600/1-76-002; PB-251 709; EPA-R-802022
Stock Number PB 251 709
OCLC Number 08181883
Subjects Oxidoreductases--physiology ; Insecticides--metabolism
Additional Subjects Insecticides ; Enzymes ; Environmental impacts ; Biodeterioration ; DDT ; Chemical reactions ; Toxicity ; In vivo analysis ; Concentration(Composition) ; Tissues(Biology) ; Oxidizers ; Metabolism ; Biochemistry ; Toxicology ; Environmental chemical substitutes ; Microsomal oxidase enzymes ; Oxidases
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 600-1-76-002 Headquarters Library/Washington,DC 01/17/2014
EKBD  EPA-600/1-76-002 Research Triangle Park Library/RTP, NC 06/06/2003
ELBD ARCHIVE EPA 600-1-76-002 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD  EPA 600-1-76-002 AWBERC Library/Cincinnati,OH 08/26/2016
NTIS  PB-251 709 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation vi, 36 pages ; 28 cm.
The role of the microsomal oxidase enzymes (MFO) in the biochemistry and toxicology of insecticides has been studied. Insects contain greatly varying titres of these enzymes. A survey of 74 species from 40 families in 8 orders, using the topical LD50 of carbaryl alone and together with the inhibitor piperonyl butoxide showed a 55,000-fold variability in LD50 largely due to MFO detoxication. In individual species of Diptera, MFO activity is highly variable with age, sex, and stage of development. The DDT-type molecule has been as a model for the study of degradophores, i.e. molecule groupings that can serve as MFO substrates. Their oxidation thus converts lipophilic compounds into more water-partitioning moieties and thus promotes excretion rather than lipid storage. Suitable degradophores for the DDT-type molecule are alkyl and alkoxy groups on the aryl rings. Compounds with judicious combinations of these provide relatively long persistence on inert surfaces and ready biodegradability in vivo. Such compounds are much less toxic to mice and to fish than DDT but because of the generally lower MFO of insects, can be effective insecticides. The role of degradophores incorporated into the aliphatic moiety of DDT has also been explored, where the -CH(CH3)2, -CHCH3Cl and -CHCH3NO2 groups are useful. Induction experiments with the biodegradable DDT analogues in mice has demonstrated that unlike DDT, these compounds do not elevate liver MFO.
"EPA-600/1-76-002." "January 1976." Includes bibliographical references (pages 31-35).