Record Display for the EPA National Library Catalog


Main Title Measurements of active biomass concentrations in biological waste treatment processes /
Author Pohland, Frederick G., ; Pohland, Frederick G., 1931-
Other Authors
Author Title of a Work
Kang, S. J.
Lewis, Ronald F.
Publisher Municipal Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency,
Year Published 1975
Report Number EPA-600/2-75-029; EPA-R-800354; EPA-ROAP-21ASR-008
Stock Number PB-246 135
OCLC Number 02459150
Subjects Dehydrogenases ; Water--Purification--Biological treatment ; Oxidoreductases
Additional Subjects Activated sludge process ; Enzymes ; Biomass ; Sewage treatment ; Bioassay ; Process control ; Escherichia coli ; Monitoring ; Aerobic processes ; Anaerobic processes ; Growth ; Oxidation ; Measurement ; Suspended solids
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 600-2-75-029 c.1 Headquarters Library/Washington,DC 06/09/2014
ELBD ARCHIVE EPA 600-2-75-029 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD RPS EPA 600-2-75-029 repository copy AWBERC Library/Cincinnati,OH 11/10/2020
ESAD  EPA 600-2-75-029 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB-246 135 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation xi, 141 pages : illustrations, charts ; 27 cm
The research was initiated to determine the applicability and limitations of the dehydrogenase test for the measurement of active biomass in biological wastewater treatment process. Pure culture with E. coli and/or heterogeneous culture batch studies were conducted on a variety of substrates including glucose, galactose, sucrose, alanine, acetic acid, and selected industrial wastewaters. Also conducted are continuous aerobic or anaerobic culture studies with and without solids recycle. Dehydrogenase activity was monitored along with other parameters including plate count, Coulter Counter enumeration, adenosine triphosphate (ATP), and suspended solids to provide comparative and complementary information on the biomass concentration. Dehydrogenase activity was a very sensitive and accurate measure of active biomass throughout the growth phases especially during endogenous growth but showed limitations with the nutrient deficient cultures. The correlation between dehydrogenase activity and suspended solids was constant at varying retention times, or at all growth rates with or without solids recycle. The technique for dehydrogenase activity measurement is simple, less costly and gives more reliable and interpretable results.
"EPA-600/2-75-029"--Cover "September 1975"--Cover "School of Civil Engineering, Georgia Institute of Technology." "Grant No. R-800354 (17050 GAI), Program Element No. 1BB043." "Project Officer Ronald F. Lewis." "Wastewater Research Division Municipal Environmental Research Laboratory." Includes bibliographical references (pages 127-134).